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Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs

The present study aimed to investigate the phytochemical and antioxidant activities of a polyherbal formulation (PHF) obtained from the Nigerian Natural Medicine Development Agency (NNMDA) using In vitro methods. Ethanolic extract of the polyhebal formulation was prepared by the Soxhlation process, Phytochemical screening, Total Phenolic Content (TPC) and Total flavonoid content (TFC) of the extracts were estimated using standard methods. Extracts were analyzed for its antioxidant potential using DPPH (1,1-diphenyl-2-picrylhydrazil), FRAP (Ferric Reducing Antioxidant Power), OH (Hydroxyl radical scavenging activity), NO (Nitric oxide scavenging activity), ABTS {2,2’-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid)}, and SRS (Superoxide Radical Scavenging activity) methods to assay their free scavenging activity. Results shows that PHF contains Flavonoids (QE) 15.61±1.95, Total phenol (GAE) 25.24±3.56, preliminary phytochemical screening revealed the presence of tannins, alkaloids, flavonoids, glycosides, Anthraquinone, Terpenoid, Steroid, Phlobatannins and Coumarins. Antioxidant study for DPPH, FRAP, OH, NO, ABTS, and SRS of PHF were 86.09±1.58, 66.95±0.03, 80.89±0.10, 68.13±0.16, 65.54±0.55, and 69.69±1.12, at 250 µg/mL respectively. When compared with known standards PHF possess high antioxidant activity in a concentration dependant manner. IC50 values of drug combination exhibited higher antioxidant potential in DPPH (26.40± 0.56 µg/mL) and ABTS (28.91±0.34 µg/mL), which may be due to the combined activity of the individual plant extracts with its high phenolic and flavonoid content. these findings may provide efficient, supportive data on the use of medicinal herbs for treatment of numerous health ailments associated with the accumulation of harmful free radicals and reactive oxygen species.

Polyherbal Formulation (PHF), Total Phenolic Content (TPC), Ferric Reducing Antioxidant Power (FRAP), 1,1-diphenyl-2-picrylhydrazil, Hydroxyl (OH) Radical Scavenging Activity

APA Style

Ahonsi Cyril Ohikhatemen, Ovobovwori Solomon Uvietobore, Etatuvie Samuel Oghene. (2023). Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs. International Journal of Homeopathy & Natural Medicines, 9(1), 6-14. https://doi.org/10.11648/j.ijhnm.20230901.12

ACS Style

Ahonsi Cyril Ohikhatemen; Ovobovwori Solomon Uvietobore; Etatuvie Samuel Oghene. Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs. Int. J. Homeopathy Nat. Med. 2023, 9(1), 6-14. doi: 10.11648/j.ijhnm.20230901.12

AMA Style

Ahonsi Cyril Ohikhatemen, Ovobovwori Solomon Uvietobore, Etatuvie Samuel Oghene. Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs. Int J Homeopathy Nat Med. 2023;9(1):6-14. doi: 10.11648/j.ijhnm.20230901.12

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1. Adeneye, A. A., Benebo, A. S. and Agbaje, E. O. (2006): Protective effect of the aqueous leaf and seed extract of phyllanthus amarus on alcohol induced hepatotoxicity in rats. West African Journal of Pharmacological Drug Research. 23: 42-50.
2. Agbo M. O.; Uzor, P. F.; Nneji, U. N. A.; Odurukwe, C. U. E.; Ogbatue, U. B.; Mbaoji, E. C (2015). Antioxidant, total phenolics and flavonoid content of selected Nigerian medicinal plants. Dhaka Univ. J. Pharm. Sci. 2015, 14, 35-41.
3. Alam, M. N., Bristi, N. J., Rafiquzzaman, M (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharma. J; 21: 143-152.
4. Akinmoladun, A. C., Obuotor, E. M. and Farombi, E. O. (2010). Evaluation of Antioxidant and Free radical scavenging capacities of some Nigerian Indigenous medicinal plants. J. Med. Food. 13 (2): 1-8.
5. Ajith T. A and. Janardhanan K. K (2007), “Indian medicinal mushrooms as a source of antioxidant and antitumor agents,” Journal of Clinical Biochemistry and Nutrition, vol. 40, no. 3, pp. 157-162.
6. Ajith, Y., Dimri, U., Dixit, S. K., Singh, S. K., Gopalakrishnan, A., Madhesh, E., Rajesh, J. B., Sangeetha, S. G. (2017). Immunomodulatory basis of antioxidant therapy and its future prospects: An appraisal. Inflammopharmacology, 25 (5), 487-498.
7. Amic D., Dusanka Davidovic-Amic, Drago Beslo, and Nenad Trinajstic (2003) Structure-Radical Scavenging Activity Relationships of Flavonoids: CROATICA CHEMICA ACTA CCACAA 76 (1) 55-61 (2003) ISSN-0011-1643 CCA-2852.
8. Arun K. Kalia A. N., Singh H (2020) Invitro antioxidant potential of polyherbal formulation of three different Herbal drugs. Indo Global Journal of Pharmaceutical sciences, 2020; 10 (4): 70-76.
9. Borar, S., Punia, P., Kalia, A. N. (2011) Antioxidant potential of n-butanol fraction from extract of Jasminum mesnyi Hance leaves. Indian J. Exp. Biol., 49: 39-43.
10. Bhakta, D. and Siva, R. (2012): Amelioration of oxidative stress in bio-membranes and macromolecules by non-toxic dye from Marinda tinctoria (Roxb) roots. Food Chemistry Toxicology. 50: 2062-9.
11. Biju, J.; Sulaiman, C. T.; Satheesh, G.; Reddy, V. R. K. (2014) Total phenolics and flavonoids in selected medicinal plants from Kerala. Int. J. Pharm. Pharm. Sci., 6, 406–408.
12. Batra, P and Sharma, A. K. (2013): Anti-cancer potential of flavonoids: recent trends and future perspectives. 3Biotech. 3 (6): 439- 459.
13. Bajpai VK, Agrawal P, Bang BH, Park YH. (2015). Phytochemical analysis antioxidant and antilipid peroxidation effects of a medicinal plant, Adhatoda vasica. J Front Life Sci; 8: 305-12.
14. Chang, C. C., Yang, M. H., Wen, H. M., Chern, J. C (2002) Estimation of total flavonoids content in propolis by two complementary colorimetric methods. J. Food Drug Anal., 10, 178-182.
15. Deepak, M. K., Surendra, S. K., Mahabaleshwar, V. H. and Hanhong, B. (2015): Significance of Antioxidant Potential of Plants and its Relevance to Therapeutic Applications. International Journal of Biological Science. 11 (8): 982–991.
16. Diplock AT (1997). Will the “Good Fairies” please prove to us that Vit E lessens human degeneration disease? Free Rad Res 1997; 27: 511- 532.
17. Das, N., Islam, M. E., Jahan, N., Islam, M. S., Khan, A., Islam, M. R. and Parvin, M. S. (2014). Antioxidant activities of ethanol extracts and fractions of Crescentia cujete leaves and stem bark and the involvement of phenolic compounds. BMC Complement Altern. Med. 14: 45.
18. Friedman, M. (2007) Overview of Antibacterial, Antitoxin, Antiviral, and Antifungal Activities of Tea Flavonoids and Teas. Molecular Nutrition & Food Research, 51, 116-134. https://doi.org/10.1002/mnfr.200600173.
19. Ghosh T, Maity TK, Das M, Bose A, Dash DK (2007). In-vitro antioxidant and Hepatoprotective activity of Ethanolic extract of Bacopa monnieri. Linn. aerial parts. Iran J Pharmacol Ther 2007; 6 (1): 77-85.
20. Gill, N. S., Bajwa, J., Sharma, P., Dhiman, K., Sood, S.(2011) Evaluation of antioxidant and antiulcer activity of traditionally consumed cucumis melo seeds. J. Pharmacol. Toxicol., 2011; 6 (1): 82-89.
21. Gupta M., Mazumder U.K and Gomathi P (2007). Antioxidant and antimicrobial properties of galega purpurea root. Asian J. Plant Sc., 6: 533-537.
22. Halliwell B, Gutteridge E (2006) Free Radicals in Biology and medicine, Ed 4. Clarendon Press Oxford.
23. Hou W. C, Lin R. D, Cheng K. T, Cho Ch Chen CH Hwang SY Lee MH (2003) free radical scanvenging activity of Taiwanese native plants Phytomed., 10: 170-175.
24. Huang DH, Chen C, Lin C, Lin Y (2005). Antioxidant and antiproliferative activities of water spinach (Ipomoea aquatica Forsk.) constituents. Bot Bull Acad Sci 2005: 46: 99-106.
25. Hamburger M., Hostettmann K (1991). Bioactivity in plants: the link between phytochemistry and medicine, Phytochemistry, 30, Issue 12, 1991, Pages 3864-3874, ISSN 0031-9422, https://doi.org/10.1016/0031-9422(91)83425-K. (https://www.sciencedirect.com/science/article/pii/003194229183425K).
26. Ilalenti A, Moncada S, Di Rosa M. (1993) Modulation of adjuvant arthritis by endogenous nitric oxide. Brit J Pharmacol 1993; 110: 701-706.
27. Jomova, K.; Vondrakova, D.; Lawson, M.; Valko, M. (2010) Metals, oxidative stress and neurodegenerative disorders. Mol. Cell. Biochem., 345, 91-104.
28. Kassuya, C. A., Silerstre, A. A., Rehder, V. and Calixto, J. B. (2003): Anti allodynic and antiedematogeni properties of lignin from Phyllanthus amarus in models of persistent inflammatory and neuropathic pain European Journal of Pharmacology, 478, 145-153.
29. Koksal, E., Gulcin, I., Beyza, S., Sarikaya, O. and Bursal, E. (2009). In vitro antioxidant activity of silymarin. J. Enz. Inh. Med. Chem. 24 (2): 395-404.
30. Kaisa, AS., Achim, M., Lenka, J., Laura, E., Korhonen, Minna, R and Risto, O. J. (2011): Inhibition of human drug metabolizing ctyochrome P450 enzymes by plant isoquinoline alkaloids. Phytomedecine: International Journal of Phytotherapy and phytopharmacology. 18 (6): 533-538.
31. Kamalakkannan N, Stanley MPP (2003). Effect of Aegle marmelos fruit extract on tissue antioxidants in streptozotocin diabetic rats. Ind J Exp Biol 2003; 41: 1285-1288.
32. Lee J. K.; You C. O.; Jin Y. N (2015) Antioxidant and antiinflamatory activity: determination of one hundred kind of pure chemicals compounds using offline and online screening HPLC Assay. Evid. Based complimentary alternat. Med. 2015; doi10.1155/2015/1654557.
33. Liu H and Visner, G. A (2008) “Oxidants and antioxidants,” Molecular Pathology of Lung Diseases, vol. 1, 2008.
34. Lillehoj. H., Liu, Y., Calsamiglia, S., Mariano, E. F., Fang, C., Ron, L. C., Sungtaek, O and Cyril, G. G. (2018): Phytochemicals as antibiotic alternatives to promote growth and enhance host health. Veterinary Research 49. 76. Doi: 10: 1186/s13567- 018-0562-6.
35. Meena H., Pandey H. K., Arya, M. C and Ahmed, Z. (2012) Evaluation of antioxidant activity of two important memory enhancing medicinal plants Baccopa monnieri and Centella asiatica. Indian Journal of Pharmacology, 44 (1), 114-117.
36. Mukhija, M., Singh, M. P., Dhar, K. L., Kalia, A. N (2015) Cytotoxic and antioxidant activity of Zanthoxylum alatum stem bark and its flavonoid constituents. J. Pharmacogn. Phytochem., 2015; 4 (4): 86-92.
37. Miyake, K., Mickley, L., Litman, T., Zhan, Z., Robey, R., Cristensen, B., Brangi, M., Greenberger, L., Dean, M., Fojo, T., Bates, S. E. (1999) Molecular cloning of cDNAs which are highly overexpressed in mitoxantrone-resistant cells: Demonstration of homology to ABC transport genes. Cancer Res.; 59 (1): 8-13.
38. Manian, R., Anusuya, N., Siddhuraju, P. and Manian, S. (2008). The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficus bengalensis L. and Ficus racemosa L. Food Chem. 107: 1000-1007.
39. Miliauskas, G., Venskutonis, P. R. and Van Beek, T. A. (2004). Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry. 85: 231-237.
40. Miguel, M. G. (2010) Antioxidant activity of medicinal and aromatic plants. A review. Flavour Frager. J., 2010; 25 (5): 291-312.
41. Moukette, B. M., Constant, A. P., Jacques, R. N., Cabral, P. N. B., Bravi, M and Jeanne, Y. N (2015): In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenols composition of a non-timber forest product used as spice: Monodora myristica. Biological Research. 45 (15): 1-17.
42. Noureddine, B. (2018): Pharmacuetical activity of alkaloids: A review. Asian Journal of Botany. (1): 1-6.
43. Okwu, D. E. and Josiah, C. (2006): Evaluation of the chemical composition of two Nigerian medicinal plants. African journal of Biotechnology. 5 (4): 357-361.
44. Okwuonu, U., Baxter-Grillo, D. C., Njoya, H. and Iyemene, P. T. (2017): Proximate and elemental constituents of Aspilia Africana (wild flower). Journal of Medicinal Plants Studies. 5 (4), 22-27.
45. Oyedemi S. O; Bradley G and Afolayan (2010) invitro and vivo antioxidant activities of Aqueous extract of Strychnos henningsii Gilg 2010 African Journal of Pharmacy and Pharmacology; 4 (2) 070-078.
46. Poonia, P., Niazi, J., Chaudhary, G., Kalia, A. N (2011). In vitro antioxidant potential of Jasminum mesnyi Hance (leaves) extracts. Res. J. Pharm. Biol. Chem. Sci.; 2 (1): 348-57.
47. Panchawat, S., Rathore, K. S., Sisodia, S. S (2010). A review on herbal antioxidants. Inter. J. Pharmtech. Res.; 2 (1): 232-239.
48. Prekumar G. R.; Sankaranarayanan S.; Jeeva and Rajarathinam k (2011) Asian J. Trop. Biomed. 1: 169-172.
49. Porto, C. D., Calligaris, S., Celloti, E. and Nicoli, M. C. (2000). Antiradical properties of commercial cognacs assessed by the DPPH test. J. Agric. Food Chemistry. 48: 4241-4245. Prakash, A. Antioxidant activity. Anal. Prog. 19: 1-6.
50. Pisoschi AM, Pop A (2015) The role of antioxidants in the chemistry of oxidative stress: a review. Eur J Med Chem 97: 55-74.
51. Rivera, J. O., Loya, A. M., & Ceballos, R. (2013). Use of herbal medicines and implications for conventional drug therapy medical sciences. Alternative & Integrative Medicine, 2 (6), 1-6.
52. Rodrigo, R., Gonzalez, J. and Paoletto, F (2011). The role of oxidative stress in the pathophysiology of hypertension. Hypertens Res 34, 431- 440.
53. Sreeramulu, D., Reddy, C. V. K., Chauhan, A., Balakrishna, N., Raghunath, M (2013). Natural antioxidant activity of commonly consumed plant foods in India: effect of domestic processing. Oxid. Med. Cell. Longev., 1-12.
54. Sen, S., Chakraborty, R., Sridhar, C., Reddy, Y. S. R., De, B. (2010) Free radicals, current status and future prospect. Int. J. Pharma. Sci. Rev. Res. 3: 91-100.
55. Sen, S., De, B., Devanna, N. and Chakraborty, R. (2013). Total phenolic, total flavonoid content, and antioxidant capacity of the leaves of Meyna spinosa Roxb., an Indian medicinal plant. Chin J. Nat. Med. 11 (2): 149-157.
56. Sagin, F. G., & Sozmen, E. Y. (2004). Anti-inflammatory effects of dietary antioxidants. Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents, 3 (1), 19-30.
57. Singh, H., Sidhu, S., Khan, M. U (2016). Free radical scavenging property of β-Aescin and Trans- chalcone: In Vitro study. Eur. J. Pharm. Med. Res.; 3 (2): 309-312.
58. Singh, H., Sidhu, S., Chopra, K., Khan, M. U (2017). The novel role of β-aescin in attenuating CCl4- induced hepatotoxicity in rats. Pharm, Biol.; 55 (1): 749-757.
59. Shirwaikar A.; Arun S.; Kuppusamy R and Isaac R. P (2006) Invitro antioxidant studies on the Benzyl tetra Isoquinoline Alkaloid berberin. Biological Pharm. Bull. 29 (9) 1906-1910.
60. Shahidi F, Wanasundara PD. (1992) Phenolic antioxidants. Cri Rev Food Sci Nutr 1992; 32 67-103.
61. Sylvie, D. D., Anatole, P. C., Cabral, B. P., Veronique, P. B. (2014). Comparison of in vitro antioxidant properties of extracts from three plants used for medical purpose in Cameroon: Acalypha raceosa, Garcinia lucida and Hymenocardia lyrata. Asian. Pac. J. Trop. Biomed., 2014; 4 (2): 625-32.
62. Spinella, M (2002). The importance of pharmacological synergy in psychoactive herbal medicines. Altern. Med. Rev., 2002; 7 (2): 130-137.
63. Spencer JPE, Jenner A, Auroma OI (1994). Intense oxidative DNA damage promoted by γ - DOPA and its metabolites, implications for neurogenerative disease. FEBS Lett 1994; 353: 246- 250.
64. Tripathi Y. B, Chaurasia S, Tripathi E, Upadhyay A, Dubey G. P (1996). Bacopa monnieri Linn as an antioxidant: Mechanism of action. Ind J Exp Biol 1996.
65. Yu, W., Zhao, Y. and Shu, B. (2004): The radical scavenging activities of radix puerariae isoflavonoids: A chemiluminescence study. Food Chemistry. 86: 525-529.
66. Wang, S. Y., Jiao, H. (2000) Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals and singlet oxygen. J. Agric. Food Chem; 48 (11): 5677-84.
67. Williams RJ Spencer JPE Rice-Evans C (2004) Flavonoid: antioxidants or signalling molecule? Free radical Biology and Medicine [internet] (cited 2023 Feb. 24); 36: 838-49 available from http://www.ncbinlm.nih.gov/pubmed/15019969