Development and Validation for HPLC Method of Assay of Lvermectin and Clorsulon in Combined Pharmaceutical Dosage Form
Mahmoud Mohamed Ali,
Elfatih Elbashir,
Mohamed N. Abdalaziz
Issue:
Volume 3, Issue 6, December 2017
Pages:
45-55
Received:
15 September 2017
Accepted:
6 November 2017
Published:
16 January 2018
Abstract: Stability indicating-HPLC method has been developed for simultaneous estimation of Ivermectin and Clorsulon in their combined dosage form. For RP-HPLC method, all the standard and sample solutions were prepared in methanol. A RP-HPLC method has been developed and subsequently validated for simultaneous estimation of Ivermectin and Clorsulon in their combination product. The proposed RP-HPLC method utilizes a Thermo BDS C-18 (15cm x 4.6mm, 5 μm) column, mobile phase consisting of acetonitrile, methanol and purified water in the proportion of 60: 30:10 (v/v/v), and UV detection at 245 nm. The described method was linear over a range of 10-40μg/ml with a correlation coefficient (r2) of 0.9998 for Ivermectin and a range of 100-400μg/ml with a correlation coefficient (r2) of 0.9998 for Clorsulon. Validations of the proposed method were carried out for its accuracy, precision, linearity and range, specificity, LOD and LOQ according to ICH guidelines. A stability-indicating study was also carried out and indicated that this method can also be used for purity and degradation evaluation of these formulations that occurred due to temperature, humidity and time. the method has been successfully applied for the analysis of drugs in formulation.
Abstract: Stability indicating-HPLC method has been developed for simultaneous estimation of Ivermectin and Clorsulon in their combined dosage form. For RP-HPLC method, all the standard and sample solutions were prepared in methanol. A RP-HPLC method has been developed and subsequently validated for simultaneous estimation of Ivermectin and Clorsulon in thei...
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