Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs
Ahonsi Cyril Ohikhatemen,
Ovobovwori Solomon Uvietobore,
Etatuvie Samuel Oghene
Issue:
Volume 9, Issue 1, June 2023
Pages:
6-14
Received:
31 January 2023
Accepted:
27 February 2023
Published:
10 June 2023
Abstract: The present study aimed to investigate the phytochemical and antioxidant activities of a polyherbal formulation (PHF) obtained from the Nigerian Natural Medicine Development Agency (NNMDA) using In vitro methods. Ethanolic extract of the polyhebal formulation was prepared by the Soxhlation process, Phytochemical screening, Total Phenolic Content (TPC) and Total flavonoid content (TFC) of the extracts were estimated using standard methods. Extracts were analyzed for its antioxidant potential using DPPH (1,1-diphenyl-2-picrylhydrazil), FRAP (Ferric Reducing Antioxidant Power), OH (Hydroxyl radical scavenging activity), NO (Nitric oxide scavenging activity), ABTS {2,2’-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid)}, and SRS (Superoxide Radical Scavenging activity) methods to assay their free scavenging activity. Results shows that PHF contains Flavonoids (QE) 15.61±1.95, Total phenol (GAE) 25.24±3.56, preliminary phytochemical screening revealed the presence of tannins, alkaloids, flavonoids, glycosides, Anthraquinone, Terpenoid, Steroid, Phlobatannins and Coumarins. Antioxidant study for DPPH, FRAP, OH, NO, ABTS, and SRS of PHF were 86.09±1.58, 66.95±0.03, 80.89±0.10, 68.13±0.16, 65.54±0.55, and 69.69±1.12, at 250 µg/mL respectively. When compared with known standards PHF possess high antioxidant activity in a concentration dependant manner. IC50 values of drug combination exhibited higher antioxidant potential in DPPH (26.40± 0.56 µg/mL) and ABTS (28.91±0.34 µg/mL), which may be due to the combined activity of the individual plant extracts with its high phenolic and flavonoid content. these findings may provide efficient, supportive data on the use of medicinal herbs for treatment of numerous health ailments associated with the accumulation of harmful free radicals and reactive oxygen species.
Abstract: The present study aimed to investigate the phytochemical and antioxidant activities of a polyherbal formulation (PHF) obtained from the Nigerian Natural Medicine Development Agency (NNMDA) using In vitro methods. Ethanolic extract of the polyhebal formulation was prepared by the Soxhlation process, Phytochemical screening, Total Phenolic Content (T...
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